Mouse Genetics
The Mouse Genetics Core Facility provides investigators the possibility of generating genetically engineered mice by transgenic and gene targeting methodologies. The core can generate, genetically manipulate, and select mouse embryonic stem (ES) cells from various genetic strains for injection into blastocysts. Alternatively, these genetically modified ES cells can be differentiated in vitro by the core facility into various cell types for investigator research. The core also offers injection of transgenes into fertilized oocytes.
The facility can advise investigators on the generation of transgenic and targeting constructs for simple knock-out/knock-in and for tissue-specific and conditional mutations as well as strategies for screening transfected ES cells and mouse mutants. In addition, the core facility performs rederivation of "dirty" mice by embryo transfer and cryopreservation of mouse strains (embryos).
Our Services
The following is a list of services commonly provided by our facility. Please direct inquiries about these services or any additional services not listed below by emailing us at [email protected]. We can readily provide you with forms, timelines, and prices for any given service. We also have an extensive collection of references and protocols compiled for your use.
Design and Generation of Constructs
We will help you develop and design constructs for your research. We make constructs for BAC transgenics, conventional transgenics, and gene targeting. We can also make expression constructs for your research.
Gene Targeting in Mouse ES Cells
Targeting mouse embryonic stem cells for a desired genetic modification.
Blastocyst Injection
Production of chimeric mice by injecting targeted ES cells into blastocyst stage embryos.
Pronuclear Injection
Production of potential transgenic founder mice by injecting a DNA construct into the pronucleus of zygotes.
Cryopreservation of Mouse Embryos
Cryopreservation and long-term storage of 8-cell stage mouse embryos in liquid nitrogen.
Rederivation of Mouse Lines
Rederivation of mouse lines from cryopreserved embryos.
Chromosome Counting
Determination of chromosome number in ES cell clones.
Mycoplasma Testing
Testing of tissue culture supernatant for the presence of mycoplasma.
