Cytometry Core Facility
303-398-1330
The cytometry core serves about 50 laboratories with more than 250 individual users at National Jewish Health, University of Colorado Denver, the Cancer Center and other outside organizations. The objectives of this core are to provide high quality state-of-the-art data to researchers at a reasonable cost and to train graduate students and post docs in the use of this technology.
Our Services
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4 cytometer analyzers
Range from a system with two lasers and four colors to a system with five lasers and eighteen colors.
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2 high speed cell sorters
Range from one way sort to four way sort and plate sorts.
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8 imaging microscope systems
Provide a range of services from a simple photograph to real time live cell imaging with multi-color 3D imaging, confocal and two photon imaging.
The cytometry staff maintain the equipment and will help users design experiments, use the equipment and analyze their data. Users are strongly encouraged to learn to operate the instruments without operator assistance. This training will help users better understand data, allow extended hours, reduce costs and achieve the greatest productivity and quality.
View all our available instruments and services.
How Our Services Help
Cytometry allows users to obtain information about individual cells in a population. Flow cytometry allows the analysis of hundreds to thousands of cells in a second, which allows good sampling statistics even for rare cell populations. Cells may be characterized by eleven or more parameters. Standard tests include immunofluoresence, DNA measurements, and ion flux measurements, such as Ca++ or pH.
Imaging systems give a lot of information about a single cell or cell-cell relationships, but do not give the high numbers obtained in flow cytometry. Most of the microscopes can obtain information in three dimension as well as multiple excitation and emission wave lengths. This allows users to generate a three dimensional view of the cell to better understand how the different parts and molecules are related. Rapid live cell imaging is possible so that several colors and several layers in a cell can be imaged in a second. This will allow experiments such as real-time tracing of molecular motions inside the cell.
Staff
